1000 Bp Dna Ladder

The ladders provide the highest level of convenience during the routine handling and avoid commonly used gel staining procedures with the ethidium bromide or sybr green i.

1000 bp dna ladder.

The 1k and 3k bands have increased intensity to serve as reference points. The 500 bp 1000 bp and 3000 bp fragment are used as reference band. The dna ladder consists of 10 dna fragments and is provided with 6x tritrack dna loading dye. Ready to use it contains loading dyes with one or two migration visualisation dyes.

It is composed by 10 fragments ranging from 100 1 000 base pairs with 100 bp increments between bands. All fragments have a 4 base 5 overhangs that can be end labeled using t4 polynucleotide kinase m0201 or filled in using dna polymerase i klenow fragment m0210 1. The 100 bp dna ladder consists of 13 double stranded molecular weight markers ranging in sizes from 100 to 1 000 bp in 100 bp increments and additional fragments of 1 200 1 600 2 000 bp. The 500 1 000 and 2 000 bp bands are two times brighter for easy identification.

There are no unspecific bands besides the fragments. Thermo scientific generuler 100 bp dna ladder is recommended for sizing and approximate quantification of a double stranded dna in the range of 100 bp to 1 000 bp on agarose or polyacrylamide gels. We recommend loading 0 5 μg of 1 kb dna ladder diluted in sample buffer. The onemark b includes fragments ranging from 250 10 000 base pairs.